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June 

Monday 1stTuesday 2ndWednesday 3rdThursday 4thFriday 5thSaturday 6thSunday 7th
Monday 8thTuesday 9thWednesday 10thThursday 11thFriday 12th Saturday 13thSunday 14th
Monday 15thTuesday 16thWednesday 17thThursday 18thFriday 19thSaturday 20th Sunday 21st
 LR    innoculate midis
 

LR hEF1a:LacI

LR pLac:mKate

LR TRE:mKate

Kill LR + transform

pick colonies

design seq. prim.

miniprep

digest

seq

check seq datainnoculate midis
  

Linker half annealing

GG: CDA-XTCSX-G4

Transform

Pick colonies

Design seq. prim.

Miniprep

Digest

Seq

check seq dataInnoculate midis
    

h:CDB-p:RFP-G4

SDM + gel

  
Monday 22ndTuesday 23rdWednesday 24thThursday 25thFriday 26thSaturday 27thSunday 28th

midiprep

transfection

add small

molecule

cytometry

data analysis

data analysisdata analysis  

Midiprep:

LR hEF1a:LacI

LR pLac:mKate

LR TRE:mKate

transfection

add small

molecule

cytometry 

data analysis

 data analysis

data analyis +

planning for

CD linker

optimization

  

Midi

 

 

     

Transform

h:CDB-p:RFP-G4

pick colonies

design seq. prim.

Miniprep

Digest

Seq

check seq data  Innoculate midis
Monday 29thTuesday 30th     
midiprep      


July

Monday Tuesday Wednesday 1stThursday 2ndFriday 3rdSaturday 4thSunday 5th
       
Monday 6thTuesday 7thWednesday 8thThursday 9thFriday 10th Saturday 11thSunday 12th
       
Monday 13thTuesday 14thWednesday 15thThursday 16thFriday 17thSaturday 18th Sunday 19th
       
Monday 20thTuesday 21stWednesday 22ndThursday 23rdFriday 24thSaturday 25thSunday 26th
       
Monday 27thTuesday 28thWednesday 29thThursday 30th Friday 30th   
       

August

Experiments

 Transfection optimization
 Dox/IPTG dose response curve
 CD79 linker optimization
 Syk linker optimization
 Expression level optimization
 Lyn or no lyn
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