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Experimental Progress:
Linker Optimization
Useful Links:
Linker optimization
| Task | Claimed by | Date Completed | Other Notes |
|---|
| CD79A/B, Gal4VP16, RFP PCR try3 | CR | Apr 10 | |
| Gel verification of try3 PCR product | LA | Apr 10 | RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers |
| Redesign primers | LA + CR | Apr 11 | CD79A F and R and CD79B F primers were redesigned and ordered |
| AarI golden gate of CD79A construct | LA | Apr 30 | |
| Take out CD79A golden gate plates | LA | May 2 | |
Make LB | CR | May 4 | |
CD79A golden gate liquid cultures | LA | May 4 | Cultures in at 6pm - need to be taken out by 10/11 am May 5 in 215 shaking incubator |
| Take out CD79A golden gate liquid cultures | LA | May 5 | In 4C |
CD79A golden gate minipreps | LA | May 6 | In yellow rack in 4C - need to be nanodropped |
CD79A golden gate restriction verification | CR | May 6 | Some promising constructs |
| Send promising CD79A golden gates for sequencing | | | |
- CD79A linker golden gates
| | | |
| Redesign CD79B reverse PCR primer | | | |
Variable region construction
| Task | Claimed by | Date Completed | Other Notes |
|---|
| Order primers for PCRing constant region | | | primers on geneious |
| | | | |
- task dependent on above task
Higher Level To Do's:
- Finish Discussing Figures for Experiment list
- Decide on other antigen-variable regions we want to test
- Syk-Lyn experiment design
- Receptor dilution experiment design
- Other experimental planning