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hEF1a:CD79A-pTet:mRFP-Gal4VP16 minipreps:
ReplicateConcentration
h:CDA-pT:RFP-G4 1 
h:CDA-pT:RFP-G4 2 
h:CDA-pT:RFP-G4 3 
h:CDA-pT:RFP-G4 4 
h:CDA-pT:RFP-G4 5 

 

hEF1a:CD79A-pTet:mRFP-Gal4VP16 restriction digest verification:

Enzyme used: BamH1

ExpectedLadderGelNotes

Lanes are Ladder,

hEF1a:CD79A-pTet:mRFP-Gal4VP16

sample 1, 2, 3, 4, 5.

Only samples 1 and 5 appear to have succeeded.

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