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BCR 2.0 

Cloning Progress

Parts Tree

Cloning Strategy

Experiments

Linker Optimization

Antibody Choices 

Immediate To Do's:
TaskClaimed byDate CompletedOther Notes
CD79A/B, Gal4VP16, RFP PCR try3CRApr 10

 

Gel verification of try3 PCR productLAApr 10RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers
Redesign primersLA + CRApr 11CD79A F and R and CD79B F primers were redesigned and ordered
AarI golden gate of CD79A constructLAApr 30 
Take out CD79A golden gate platesLA  
  • Make LB
   
  • CD79A golden gate liquid cultures
   
  • CD79A golden gate minipreps
   
  • CD79A golden gate restriction verification
   
Redesign CD79B reverse PCR primer   

 

  • task dependent on above task
Higher Level To Do's:
  • Finish Discussing Figures for Experiment list
  • Decide on other antigen-variable regions we want to test 
  • Syk-Lyn experiment design 
  • Receptor dilution experiment design
  • Other experimental planning

Useful Links 

Protocols       
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