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BCR 2.0 

Cloning Progress

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Cloning Strategy

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Things to do:

TaskClaimed byDate CompletedOther Notes
CD79A/B, Gal4VP16, RFP PCR try3CRApr 10

 

Gel verification of try3 PCR productLAApr 10RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers
Redesign primersLA + CRApr 11CD79A F and R and CD79B F primers were redesigned and ordered
CD79s PCR with new primers   
Gel verification of CD79 PCRs with new primers   
AarI golden gates   

To Do:

  • Redesign CD79B PCR primers
  • AarI Golden GateCD79A-pTet:RFP-Gal4VP16
  • Gel Extraction of Q1-CD79A-Q3
  • Column Purification of pTet-RFP and Gal4VP16 PCR products
  • Digest & Gel of CD79A-pTet: RFP - Gal4VP16
  • Establish Cryostocks of GmabH, GmabL, Syk-Tev, and Lyn-Tev pEXPRs and dilute DNA to 200 ng/uL

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