Construct | Temperature |
|---|---|
| CD79A | |
| CD79B | |
| Gal4VP16 | |
| pTEt:mRFP |
| Construct | Expected Band Size |
|---|---|
| CD79A | 750 bp |
| CD79B | 750 bp |
| Gal4VP16 | 750 bp |
| pTet:mRFP | 800 bp |
| Ladder | CD79A | CD79B | Gal4VP16 | pTet:mRFP |
 |  |
Notes:
Gal4VP16 constructs have the correct band size --> ready for AarI golden gate
CD79A and CD79B PCRs still seem to have primer dimers. CD79A/B primers have to be redesigned.