You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 9 Next »

THINGS TO DO 

Experiments 

TaskPersonDate

Syk-TEVp cleavage with serial dilutions

    • Transfect
    • Add dox
    • Cytometry (1.30pm)

CR 

 

 

  • 8/26
  • 8/27
  • 8/28
BCR membrane localization with serial dilution CR 

Cofilin Western Blot

    • Run gel and trasnfer
    • Antibody incubations and imaging
LA

 

  • 8/26
  • 8/27

PirB abeta binding live microscopy

    • Stain and prepare for microscopy (5pm)
LA 

 

  • 8/27

LilrB2 localization cytometry

  • Transfect
  • Stain and prepare for cytometry (1.30 pm)
LA

 

  • 8/26
  • 8/28

LilrB2 abeta binding cytometry

  • Transfect
  • Add dox
  • Stain and prepare for cytometry (1.30 pm)
LA

 

  • 8/26
  • 8/26
  • 8/28

TEVp-Cofilin cleavage

  • Transfect
  • Prepare for cytometry (1.30 pm)
LA 
  • 8/26
  • 8/28
L7ae dilution seriesGB 
BACE1 and BACE2 localizationJA 
BACE1 and BACE2 assayJA 

 

Data Analysis

Experiment to be AnalyzedPerson
miRNA L7Ae Sensors (w w/out siRNA)JX, GB
  
  
  
  

 

 

Cloning

TaskPersonDate
Lyn-TEVp  

BACE1 YFP fusion

    • LR
    • Kill and transform
    • Pick colonies
    • Miniprep
    • Verify pEXPR
    • Midiprep
  

BACE2 YFP fusion

    • LR
    • Kill and transform
    • Pick colonies
    • Miniprep
    • Verify pEXPR
    • Midiprep
  
BCR Gibson  
LilrB2-YFPSS 
LilrB2-TCS-Gal4VP16SS 

Non-Experimental Stuff 

TaskPersonDate
External WikiTsar: ?
JX (willing to help)		 
Survey analysis  
Novartis person (what do we want from her?)  

 

 

 

  • No labels