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Todo Today:

  • Miniprep LRs
  • Digest LRs
  • Also digest pEXPR_hEF1a(tongue)irBYFP
  • Transform PirB-YFP LRs (maybe do treatment's one too...)
  • PCR LilrB2 for YFP and TCSG4
  • Run: Digests, PCRs
  • Cut out PCR bands if worked.

PCRing at 53 degress, 40 seconds.

Digesting with:

pEXPR_hEF1a: LilrB2 XhoI, CS
pEXPR_TREt: LilrB2 XbaI, CS
pEXPR_hEF1a: PirB ApaI, CS


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