Controls:
Well 2: Bleed through from eBFP into mKate
Well 3: Leaky expression of Gal4UAS:mKate without the presence of Gal4VP16
Well 4: Background activation of Gal4UAS:mKate without presence of TEVp-Cofilin
Well 1HEK293 | Well 2HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Dummy 200ng | Well 3HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng | Well 4HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate 200ng Dummy 200ng | Well 5
| Well 6
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Well 7HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 0nM DOX | Well 8HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 1nM DOX | Well 9HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 10nM DOX | Well 10HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 100nM DOX | Well 11HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 1000nM DOX | Well 12HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 2000nM DOX |
Well 13HEK293 | Well 14HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Dummy 200ng | Well 15HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng | Well 16HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate 200ng Dummy 200ng | Well 17
| Well 18
|
Well 19HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 0nM DOX | Well 20HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 1nM DOX | Well 21HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 10nM DOX | Well 22HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 100nM DOX | Well 23HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 1000nM DOX | Well 24HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 2000nM DOX |