- Miniprep pEXPR (hEF1a: PirBTCS, hEF1a: TEVpCofilin)
- Miniprep pENTR LilrB2TCS
- Verify pENTR LilrB2TCS
- Midiprep inoculate hEF1a:TCS
- Pick more TREt:TEVpCofilin
- Plate more TREt:TEVpCofilin if possible.
- Transform more TREt:TEVpCofilin LR
Digest pEXPR_hEF1a: PirBTCSG4VP16 with BsrGI, buffer 2.1
Digest pENTR_LilrB2:TCSG4VP16 with XhoI +XbaI, buffer CS