To Do:
- Miniprep PY, LY, pENTR_TEVpCofilin
- Verify Minipreps
- Transform hEF1a:YFPCofilin
- Pick TREt:YFPCofilin Colonies
- LR pENTR_TEVpCofilin
3/4 pENTR_TEVpCofilin worked!
Gel
Of course, the others didn't work. Given the lack of blue colonies, I'm thinking backbone religation/only one part present (probably YFP). Need to figure out something else.