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To Do:

  • Miniprep PY, LY, pENTR_TEVpCofilin
  • Verify Minipreps
  • Transform hEF1a:YFPCofilin
  • Pick TREt:YFPCofilin Colonies
  • LR pENTR_TEVpCofilin

 

3/4 pENTR_TEVpCofilin worked!

Gel

 

Of course, the others didn't work. Given the lack of blue colonies, I'm thinking backbone religation/only one part present (probably YFP). Need to figure out something else.

 

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