Membrane Localization of Lyn

Testing where in the cell Lyn is.  This test may be a debug, if it doesn't localize, if may be because it doesn't need to associate with CD79A/B for very long to phosphorylate it, it may drift over to CD79A/B, phosphorylate it, then leave.  A failure at this test may indicate the reason for a failure of Syk localization, but it does not indicate a problem with the BCR on its own.

To determine if our circuit is assembling the way we expect it to and to see if Lyn recruitment is dependent on activation (which could mean that Lyn recruitment be used as a mechanism for signal transduction).

BCR (CD79A/B, IgM heavy, IgM light), Lyn-mKate

anti IgM

Produce two samples of cells with all parts, anti IgM +/- and look at with a microscope

Lyn should be membrane localized.  We will be lucky if Lyn only membrane localizes in anti IgM+ samples.