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Peripheral Experiments:
Inducible and Constitutive Cascades
| Number | Name | Parts/Reagents |
|---|
| 1 | Membrane Localization of BCR | Parts/Reagents - B Cells
- hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- Cover Slips
- 0.1% Gelatin
- RPMI1640
- Sodium Azide
- Fixation Buffer
- Parafilm
- Triton X-100
- Embedding Solution
- Anti IgM Green
|
| 2 | Beta Amyloid Binding of Gmab in BCR Complex | Parts/Reagents - hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- Cover Slips
- 0.1% Gelatin
- Sodium Azide
- Fixation Buffer
- Parafilm
- Triton X-100
- Embedding Solution
- Biotinylated aBeta
- Streptavidin-RED
|
| 3 | Syk Western Blot | |
| 4 | Membrane Localization of Lyn | Parts/Reagents - hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- TRE: Lyn-mKate
- hEF1a: rtTA
- Dox
- Biotinylated aBeta
|
| 5 | Membrane Localization of Syk | Parts/Reagents - hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- hEF1a: Lyn
- TRE: Syk-eYFP
- hEF1a: rtTA
- Biotinylated aBeta
|
| 6 | Inactive TANGO Test | Parts/Reagents - hEF1a: Gmab H
- hEF1a: Gmab L
- hEF1a: CD79A-TCS-Gal4VP16
- hEF1a: CD79B-TCS-Gal4VP16
- hEF1a: Lyn
- TRE: Lyn-TEV
- TRE: Syk-TEV
- hEF1a: rtTA
- UAS: eYFP
- Dox
|
| 7 | Active TANGO Test | Parts/Reagents - hEF1a: Gmab H
- hEF1a: Gmab L
- hEF1a: CD79A-TCS-Gal4VP16
- hEF1a: CD79B-TCS-Gal4VP16
- hEF1a: Lyn
- TRE: Lyn-TEV
- TRE: Syk-TEV
- hEF1a: rtTA
- UAS: eYFP
- Biotinylated aBeta
- Anti IgM Green
|