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TRANSFECTION PLANS
L7ae Repression
Name | Location | Concentration |
MAV1212-hEF1a-eBFP2 | TC Room | Midi prepped |
pEXPR:hEF1a-L7ae | TC Room | Midi prepped |
pEXPR: CAG-2xKturn-eGFP (wt) | TC Room | Midi prepped |
pEXPR: CAG-exKturn-eGFP (mut) | TC Room | Midi prepped |
EXPERIMENTS
- hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
- Should be yellow and blue
- hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
- Should be blue (minimal yellow)
![](/confluence/download/attachments/98630593/001.jpg?version=1&modificationDate=1404743480000&api=v2)
TAL14 Repression
Name | Location | Concentration |
pEXPR: hEF1a-eBFP2 | TC Room | Midi prepped |
pEXPR: hEF1a-Gal4VP16 | Our -20 | Verified |
pEXPR: hEF1a-TAL14 | Our -20 | Mini prepped (unverified, have liquid culture) |
pEXPR: TAL14-mKate | Our -20 | Midi prepped (diluting and moving to TC room) |
EXPERIMENTS:
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
- negative control (minimal yellow)
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
- positive control (hopefully linear BFP/YFP)
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14
- Should be blue (minimal yellow)
![](/confluence/download/attachments/98630593/2014-07-07%2009.33.38.jpg?version=3&modificationDate=1404743031000&api=v2)