Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- Why didn't the oligos nanodrop??
- Why didn't the primers bind to LS_miR_144?
miRNA Sensors (Lab Work)
- Golden Gate
- Low Sensor miR-144-miR-181c
- Transform
- High Sensor miR-30d (I13 & J13)
- High Sensor miR-146a (I5 & J5)
- High Sensor let-7f (1.13 & 1.14)
- High Sensor miR-125b (A3 & B3)
- Low Sensor miR-144 (E5 & F5)
- Low Sensor miR-181c (G7 & H7)
- Pick colonies to miniprep
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- FACS cell prep
- Transfect L7ae Experiment
NOTES