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TRANSFECTION PLANS
L7ae Repression
Name | Location | Concentration |
MAV1212-hEF1a-eBFP2 | Our -20 | Grown in midi culture |
pEXPR:hEF1a-L7ae | Our -20 | Grown in midi culture |
pEXPR: hEF1a-2x Kturn-eYFP | Our -20 | Grown in midi culture |
EXPERIMENTS
- hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
- Should be yellow and blue
- hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
- Should be blue (minimal yellow)
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TAL14 Repression
Name | Location | Concentration |
pEXPR: hEF1a-eBFP2 | Our -20 | Grown in midi culture |
pEXPR: hEF1a-Gal4Vp16 | With Jin? Brian? | Still looking |
pEXPR: hEF1a-TAL14 | Out -20 | Grown in midi culture |
pEXPR: 5xUAS/4xTAL14bs-eYFP | With Brian? | UAS needs to be added somehow? |
EXPERIMENTS:
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
- negative control (minimal yellow)
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
- positive control (hopefully linear BFP/YFP)
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14
- Should be blue (minimal yellow)
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