Test using the whole detection circuit and Beta Amyloid to measure circuits response to input
Purpose
This will determine the ideal concentration of Syk-TEVp for the best signal/noise ratio as well as characterize our circuit for use in other circuits.
Parts Needed
BCR (CD79A+B, IgM heavy, IgM light), Lyn, Syk-TEVp, (reporter circuit), (whatever is needed to make dox work). We will be testing all combinations of CD79(A/B)-(stop/TCS-Gal4Vp16).
Dox, Beta Amyloid
Setup
All combinations of plasmids being tested will be prepared and put into dox ladders
Expected Results
We should identify what level of Syk-TEVp causes constitutive expression of the reporter and characterize the off behavior of our circuit so we will be able to compare it to the on behavior later.