PCR of Gmab Heavy

Gel shows successful PCR of Gmab Heavy from gBlock mIgM Gmab Heavy.

Forward primer: gBlock F

Reverse primer: gBlock R

Annealing temperature: ?

Run using samples after PCR purification. Extra bands indicated multiple PCR products, used gel extraction to purify out the appropriate band.

Restriction Digest Verification of pENTR

7 July 2014:

See gel here

Correct plasmids

Failed plasmids

Gmab Heavy (1)

Gmab Heavy (2)

Gmab Heavy (3)

Gmab Heavy (4)