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CONSTRUCTION
MAV1212-Hef1a-eBFP2MAV1212-Hef1a-mKate2
  • Obtain
  • Gateway together
  • Golden Gate in Target Sites
  • Golden Gate in Target Sites
TESTING
  • Obtain siRNA sequences
  • Obtain HEK293 cells
  • Transfect siRNA into HEK cells
  • Transfect our circuit into HEK cells
  • Measure fluorescence with flow cytometry
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