| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Update experiments page
- Obtain plasmid maps
- Email Jin about TAL14 and miRNA target sites
- Talk to Lyla and Alexa about transfection schedule
- Look for plasmids
- UAS-Gal4-TAL14-eYFP
- pEXPR: Gal4VP16
- pEXPR: Hef1a-TAL14
- pEXPR: Hef1a-2x Kturn-eYFP
miRNA Sensors (Lab Work)
- Transformations
- Low Sensor Golden Gates
- pEXPR: Hef1a-eBFP2 (186 ng/uL)
- pEXPR: Hef1a-L7ae (96 ng/uL)
- Pick red colonies and grow in medium!
- Find concentrations of eBFP2 and L7ae
NOTES
Nelson doesn't know where it is, but he needs it as well, so he's on the hunt