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NEED TO TEST
Goal: Test the strength of different repressors
| Name | On | Off | Inducible |
|---|
| L7ae | - Gateway Hef1a-2x Kturn-eYFP with pENTR_L1_L7ae_L2
- Co transfect w/ constituative eBFP2
- Flow Cytometry and analysis
| - Just Hef1a-2x Kturn-eYFP
- Co transfect w/ constituative eBFP2
- Flow Cytometry and analysis
|
|
| TAL14 | - Gateway Hef1a-2x Kturn-eYFP with pENTR_L1_TAL14_L2
- Co transfect w/ constituative eBFP2
- Flow Cytometry and analysis
| - Just Hef1a-2x Kturn-eYFP
- Co transfect w/ constituative eBFP2
- Flow Cytometry and analysis
|
|
Notes:
2x-Kturn-eYFP from Nelson