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Filippo

Species	Strain
E.coli (background K12) --> BL1	HCB33 (TSS496): E. coli wild type HCB1584: E. coli D(tar) HCB268: E. coli tsr::Tn5-1a TSS945: E. coli D(tsr) UU1250: D(tar tap) (tsr::Kan) D(trg) D(aer) UU2162: D(tar tap) D(tsr) D(trg) D(aer) TSS584: HCB33 / pVS129 (ecfp on pTrc99A) TSS585: HCB33 / pVS132 (eyfp on pTrc99A) TSS586: HCB33 / mCherry on pTrc99A
E. coli (background DH5a) --> BL1	TSS379 TSS342 TSS270 TSS712 TSS711 TSS943 TSS694 HCB1666
Salmonella (background LT2) --> BL2 (attenuated)	LT2 (TSS495): Salmonella LT2 wild type LT2 (TSS497): Salmonella LT2wild type pSLT cured TSS511: Salmonella LT2 D(cheV) TSS642 (TH9972): Salmonella LT2 aer-6508::MudJ TSS505: Salmonella LT2 D(cheY cheZ) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS516: Salmonella LT2 D(cheY cheZ) D(cheV) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS869 Salmonella LT2 D(cheY cheZ) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS879 Salmonella LT2 D(cheY cheZ) D(tar) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS891: Salmonella LT2 D(cheY cheZ) D(tar) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS953: Salmonella LT2 D(cheY cheZ) D(mcpB) D(mcpC) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS954: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pML18 (tarSt) TSS880: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pVS123 (tarEc) LT2 RecA: strain with the recA gene inactivated. kanamycin resistance is inserted in the mutated site. LT2 recAo: strain with the SOS box mutated. Chloramphenicol resistance is inserted in the mutated site. LT2 LexA(Ind-): strain with the lexA gene mutated. Chloramphenicol resistance is inserted in the mutated site. LT2 CheY: strain with the cheY gene inactivated. Chloramphenicol resistance is inserted in the mutated site. LT2 CheB: strain with the cheB gene inactivated. Chloramphenicol resistance is inserted in the mutated site. TSS587: TSS497 / pVS129 (ecfp on pTrc99A) TSS588: TSS497 / pVS132 (eyfp on pTrc99A) TSS589: TSS497 / mCherry on pTrc99A
Salmonella (background Salmonella 14028) --> BL2	MB211 (TSS871): Salmonella 14028 Δtsr Δtar Δtrg Δaer ΔmcpB ΔmcpC Δtcp ΔmcpA Δtip
Escherichia coli (WT)	RP437 (from Linda Turner, Berg's Lab)

Melissa

Name	Strain	Media to use	Location
Vibrio coralliilyticus	BAA-450 (directly from ATCC; used for ISME J 2014 paper)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	P1 GFP (from Joe Pollock/David Bourne)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	YB1 wild type (from Eugene Rosenberg by way of Orr Shapiro/Assaf Vardi-- in principle should be same as ATTC type strain)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	YB2 wild type (from Eugene Rosenberg by way of Orr Shapiro/Assaf Vardi-- in principle should be same as ATTC type strain)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	YB1 dsRed (from Orr Shapiro/Assaf Vardi)	2216 + 50ug/ml Kan	Stocker 01
Vibrio coralliilyticus	YB2 dsRed (from Orr Shapiro/Assaf Vardi)	2216 + 50ug/ml Kan	Stocker 01
Vibrio coralliilyticus	Non-motile (fla knockout) YB2 (from Dalit Meron-- sent to Rory while in Stocker lab)	2216 + 100ug/ml Kan (though 50ug should be OK too-- they were originally isolated on 200ug/ml selective medium)	Rory&Mel's box
Vibrio fortis	isolated from healthy coral (non-pathogenic; from Rory Welsh/Becky Vega-Thurber)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Rory&Mel's box
Serratia marcescens	coral pathogen from Katie Patternson's lab via Rory/Becky	2216	Rory&Mel's box
Bacteriovorax	isolated from healthy coral (preys on V.cor and V.fortis; from Rory Welsh/Becky Vega-Thurber)	grows on lawn or culture of vibrio (coralliilyticus or fortis)	Rory&Mel's box
Pseudovibrio sp.	isolated from healthy coral by JB Raina. Produces the antimicrobial compound TDA	2216	Stocker 01

Cherry

Ruegeria pomeroyi DSS-3 strain, in 2216 media, from Mary Ann Moran's lab (March 2014)

Yutaka

| | | Name | Strain || B | 1 | Vibrio sp. | 12B9 GFP || B | 2 | Vibrio sp. | 12B9RFP || B | 3 | Vibrio cycrotrophicus | 1F53 GFP || B | 4 | Vibrio cycrotrophicus | 1F53 RFP || B | 5 | Vibrio cycrotrophicus | 1F111 GFP || B | 6 | Vibrio cycrotrophicus | 1F111 RFP || B | 7 | Vibrio sp. | ZF240 GFP || B | 8 | Vibrio sp. | ZF240 RFP || B | 9 | | || C | 1 | Vibrio crassostreae | 13D11 GFP || C | 2 | Vibrio crassostreae | 13D11 RFP || C | 3 | Vibrio sp. | ZF223 GFP || C | 4 | Vibrio sp. | ZF223 RFP || C | 5 | Vibrio cycrotrophicus | 1F273 RFP || C | 6 | | || C | 7 | | || C | 8 | | || C | 9 | | || D | 1 | Vibrio cycrotrophicus | ZF65 || D | 2 | Vibrio cycrotrophicus | ZF270 || D | 3 | Vibrio ordalii | FS144 || D | 4 | Vibrio ordalii | FF93 || D | 5 | Vibrio ordalii | 1S257 || D | 6 | Vibrio ordalii | 12B9 || D | 7 | Vibrio cycrotrophicus | 1F273 || D | 8 | Vibrio cycrotrophicus | 1F175 || D | 9 | Vibrio cycrotrophicus | 1F111 || E | 1 | Vibrio cycrotrophicus | ZF14 || E | 2 | Vibrio cycrotrophicus | 1F289 || E | 3 | Vibrio cycrotrophicus | 1F289 || E | 4 | V. alginolyticus | YM4 |Melissa

BOX2

A
1
Vibrio cycrotrophicus
1F111
hsp60 confirmed
A
2
Vibrio cycrotrophicus
1F175

A
3
Vibrio cycrotrophicus
1F273
hsp60 confirmed
A
4
Vibrio cycrotrophicus
1F289
hsp60 confirmed
A
5
Vibrio cycrotrophicus
1F53
hsp60 confirmed
A
6
Vibrio cycrotrophicus
1F97
hsp60 confirmed
A
7
Vibrio cycrotrophicus
FF160

A
8
Vibrio cycrotrophicus
FF274

A
9
Vibrio cycrotrophicus
FFA

B
1

B
2
Vibrio cycrotrophicus
ZF14
hsp60 confirmed
B
3
Vibrio cycrotrophicus
ZF205

B
4
Vibrio cycrotrophicus
ZF270
hsp60 confirmed
B
5
Vibrio cycrotrophicus
ZF28

B
6
Vibrio cycrotrophicus
ZF30
hsp60 confirmed
B
7
Vibrio cycrotrophicus
ZF65
hsp60 confirmed
B
8
Vibrio cycrotrophicus
ZF99

B
9

Box 01
Strain
B
1
Vibrio sp.
12B9 GFP
B
2
Vibrio sp.
12B9RFP
B
3
Vibrio cycrotrophicus
1F53 GFP
B
4
Vibrio cycrotrophicus
1F53 RFP
B
5
Vibrio cycrotrophicus
1F111 GFP
B
6
Vibrio cycrotrophicus
1F111 RFP
B
7
Vibrio cycrotrophicus
ZF270 GFP
B
8
Vibrio cycrotrophicus
ZF270 RFP
B
9

C
1
Vibrio crassostreae
13D11 GFP
C
2
Vibrio crassostreae
13D11 RFP
C
3
Vibrio sp.
ZF223 GFP
C
4
Vibrio sp.
ZF223 RFP
C
5
Vibrio cycrotrophicus
FF274 RFP
C
6

C
7

C
8

C
9

D
1
Vibrio cycrotrophicus
ZF65
D
2
Vibrio cycrotrophicus
ZF270
D
3
Vibrio ordalii
FS144
D
4
Vibrio ordalii
FF93
D
5
Vibrio ordalii
1S257
D
6
Vibrio ordalii
12B9
D
7
(Vibrio cycrotrophicus)
(1F273)
D
8
(Vibrio cycrotrophicus)
(1F175)
D
9
Vibrio cycrotrophicus
1F111
E
1
Vibrio cycrotrophicus
ZF14
E
2
(Vibrio cycrotrophicus)
(1F289)
E
3
(Vibrio cycrotrophicus)
(1F289)
E
4
V. alginolyticus
YM4
E
5
Vibrio cycrotrophicus
ZF30
E
6
Vibrio cycrotrophicus
ZF99
E
7
Vibrio cycrotrophicus
ZF205
E
8
Vibrio cycrotrophicus
ZF28
E
9
Vibrio cycrotrophicus
FF160
F
1
Vibrio cycrotrophicus
1F53
F
2
Vibrio cycrotrophicus
1F97
F
3
Vibrio cycrotrophicus
FF160
F
4

F
5

F
6

F
7

F
8

F
9

F

E.coli (background K12) --> BL1
HCB33 (TSS496): E. coli wild type
HCB1584: E. coli D(tar)
HCB268: E. coli tsr::Tn5-1a
TSS945: E. coli D(tsr)
UU1250: D(tar tap) (tsr::Kan) D(trg) D(aer)
UU2162: D(tar tap) D(tsr) D(trg) D(aer)

TSS584: HCB33 / pVS129 (ecfp on pTrc99A)
TSS585: HCB33 / pVS132 (eyfp on pTrc99A)
TSS586: HCB33 / mCherry on pTrc99A

E. coli (background DH5a) --> BL1
TSS379
TSS342
TSS270
TSS712
TSS711
TSS943
TSS694
HCB1666

E. coli (natural isolates) --> BL2
ECOR2
ECOR12
ECOR13
ECOR18
ECOR19
ECOR21
ECOR27
ECOR29
ECOR31
ECOR32
ECOR33
ECOR37
ECOR45
ECOR48
ECOR50
ECOR51
ECOR62
ECOR68

Salmonella (background LT2) --> BL2 (attenuated)
LT2 (TSS495): Salmonella LT2 wild type
LT2 (TSS497): Salmonella LT2wild type pSLT cured
TSS511: Salmonella LT2 D(cheV)
TSS642 (TH9972): Salmonella LT2 aer-6508::MudJ
TSS505: Salmonella LT2 D(cheY cheZ) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS516: Salmonella LT2 D(cheY cheZ) D(cheV) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS869 Salmonella LT2 D(cheY cheZ) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS879 Salmonella LT2 D(cheY cheZ) D(tar) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS891: Salmonella LT2 D(cheY cheZ) D(tar) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS953: Salmonella LT2 D(cheY cheZ) D(mcpB) D(mcpC) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS954: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pML18 (tarSt)
TSS880: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pVS123 (tarEc)

TSS587: TSS497 / pVS129 (ecfp on pTrc99A)
TSS588: TSS497 / pVS132 (eyfp on pTrc99A)
TSS589: TSS497 / mCherry on pTrc99A

Salmonella (background Salmonella 14028) --> BL2
MB211 (TSS871): Salmonella 14028 Δtsr Δtar Δtrg Δaer ΔmcpB ΔmcpC Δtcp ΔmcpA Δtip

Name
Strain

Vibrio corallilyticus
BAA-450 (directly from ATCC)

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Key

Filippo

Melissa

Cherry

Yutaka

		BOX2
A	1	Vibrio cycrotrophicus	1F111	hsp60 confirmed
A	2	Vibrio cycrotrophicus	1F175
A	3	Vibrio cycrotrophicus	1F273	hsp60 confirmed
A	4	Vibrio cycrotrophicus	1F289	hsp60 confirmed
A	5	Vibrio cycrotrophicus	1F53	hsp60 confirmed
A	6	Vibrio cycrotrophicus	1F97	hsp60 confirmed
A	7	Vibrio cycrotrophicus	FF160
A	8	Vibrio cycrotrophicus	FF274
A	9	Vibrio cycrotrophicus	FFA
B	1
B	2	Vibrio cycrotrophicus	ZF14	hsp60 confirmed
B	3	Vibrio cycrotrophicus	ZF205
B	4	Vibrio cycrotrophicus	ZF270	hsp60 confirmed
B	5	Vibrio cycrotrophicus	ZF28
B	6	Vibrio cycrotrophicus	ZF30	hsp60 confirmed
B	7	Vibrio cycrotrophicus	ZF65	hsp60 confirmed
B	8	Vibrio cycrotrophicus	ZF99
B	9

		Box 01	Strain
B	1	Vibrio sp.	12B9 GFP
B	2	Vibrio sp.	12B9RFP
B	3	Vibrio cycrotrophicus	1F53 GFP
B	4	Vibrio cycrotrophicus	1F53 RFP
B	5	Vibrio cycrotrophicus	1F111 GFP
B	6	Vibrio cycrotrophicus	1F111 RFP
B	7	Vibrio cycrotrophicus	ZF270 GFP
B	8	Vibrio cycrotrophicus	ZF270 RFP
B	9
C	1	Vibrio crassostreae	13D11 GFP
C	2	Vibrio crassostreae	13D11 RFP
C	3	Vibrio sp.	ZF223 GFP
C	4	Vibrio sp.	ZF223 RFP
C	5	Vibrio cycrotrophicus	FF274 RFP
C	6
C	7
C	8
C	9
D	1	Vibrio cycrotrophicus	ZF65
D	2	Vibrio cycrotrophicus	ZF270
D	3	Vibrio ordalii	FS144
D	4	Vibrio ordalii	FF93
D	5	Vibrio ordalii	1S257
D	6	Vibrio ordalii	12B9
D	7	(Vibrio cycrotrophicus)	(1F273)
D	8	(Vibrio cycrotrophicus)	(1F175)
D	9	Vibrio cycrotrophicus	1F111
E	1	Vibrio cycrotrophicus	ZF14
E	2	(Vibrio cycrotrophicus)	(1F289)
E	3	(Vibrio cycrotrophicus)	(1F289)
E	4	V. alginolyticus	YM4
E	5	Vibrio cycrotrophicus	ZF30
E	6	Vibrio cycrotrophicus	ZF99
E	7	Vibrio cycrotrophicus	ZF205
E	8	Vibrio cycrotrophicus	ZF28
E	9	Vibrio cycrotrophicus	FF160
F	1	Vibrio cycrotrophicus	1F53
F	2	Vibrio cycrotrophicus	1F97
F	3	Vibrio cycrotrophicus	FF160
F	4
F	5
F	6
F	7
F	8
F	9
F

	Name	Strain
E.coli (background K12) --> BL1
HCB33 (TSS496): E. coli wild type
HCB1584: E. coli D(tar)
HCB268: E. coli tsr::Tn5-1a
TSS945: E. coli D(tsr)
UU1250: D(tar tap) (tsr::Kan) D(trg) D(aer)
UU2162: D(tar tap) D(tsr) D(trg) D(aer)

TSS584: HCB33 / pVS129 (ecfp on pTrc99A)
TSS585: HCB33 / pVS132 (eyfp on pTrc99A)
TSS586: HCB33 / mCherry on pTrc99A

E. coli (background DH5a) --> BL1
TSS379
TSS342
TSS270
TSS712
TSS711
TSS943
TSS694
HCB1666

E. coli (natural isolates) --> BL2
ECOR2
ECOR12
ECOR13
ECOR18
ECOR19
ECOR21
ECOR27
ECOR29
ECOR31
ECOR32
ECOR33
ECOR37
ECOR45
ECOR48
ECOR50
ECOR51
ECOR62
ECOR68

Salmonella (background LT2) --> BL2 (attenuated)
LT2 (TSS495): Salmonella LT2 wild type
LT2 (TSS497): Salmonella LT2wild type pSLT cured
TSS511: Salmonella LT2 D(cheV)
TSS642 (TH9972): Salmonella LT2 aer-6508::MudJ
TSS505: Salmonella LT2 D(cheY cheZ) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS516: Salmonella LT2 D(cheY cheZ) D(cheV) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS869 Salmonella LT2 D(cheY cheZ) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS879 Salmonella LT2 D(cheY cheZ) D(tar) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS891: Salmonella LT2 D(cheY cheZ) D(tar) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS953: Salmonella LT2 D(cheY cheZ) D(mcpB) D(mcpC) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS954: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pML18 (tarSt)
TSS880: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pVS123 (tarEc)

TSS587: TSS497 / pVS129 (ecfp on pTrc99A)
TSS588: TSS497 / pVS132 (eyfp on pTrc99A)
TSS589: TSS497 / mCherry on pTrc99A

Salmonella (background Salmonella 14028) --> BL2
MB211 (TSS871): Salmonella 14028 Δtsr Δtar Δtrg Δaer ΔmcpB ΔmcpC Δtcp ΔmcpA Δtip
	Vibrio corallilyticus	BAA-450 (directly from ATCC)