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Results

 

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tubeHyper LadderGG DONRpENTRSyk-eYFP 1pENTRSyk-eYFP 2pENTRSyk-eYFP 3pENTRSyk-eYFP 4pENTR Syk tev 1pENTR Syk tev 2pENTR Syk tev 3pENTR Syk tev 4Syk+stop 1Syk+stop 2Syk+stop 3Syk+stop 4Syk+stop 5pENTR Lyn TEV 1pENTR Lyn TEV 2pENTR Lyn TEV 3pENTR Lyn TEV 4Hyper Ladder
enzyme NsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiIPvuIIPvuIIPvuIIPvuII 
Result(P/F)  FFFFPPPFFPFFFFFFF 
Notes  looks like Q to Q ligation

Plasmids

NamepENTR CD79A+STOPpENTR CD79A-TCS-Gal4VP16pENTR Syk-eYFPpENTR Syk+STOPpENTR Syk-TEVpENTR Lyn-TEVpENTR Lyn+STOPpENTR Lyn-mKate
virtual gelImage RemovedImage RemovedImage RemovedImage RemovedImage RemovedImage RemovedImage RemovedImage Removed
selected enzyme        
   
Tubeenzymeresult
   
              ggDONRggDONR ggDONRggDONR  
      
Lane 21 2223 24 2526 27 2829 30 3132 33 3435 36 3738 39 40
 Tube Hyper Ladder
   
   
   
   

 

GEL

GG DONRpENTR Lyn-mKate 1pENTR Lyn-mKate 2pENTR Lyn-mKate 3pENTR Lyn-mKate 4pENTR Lyn-stop 1pENTR Lyn-stop 2pENTR Lyn-stop 3pENTR Lyn-stop 4CD79A-tcs-gal4 1CD79A-tcs-gal4 2CD79A-tcs-gal4 3CD79A-tcs-gal4 4CD79A-tcs-gal4 5CD79A-stop 1CD79A-stop 2CD79A-stop 3CD79A-stop 4CD79A-stop 5
Enzyme PvuIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuII
Result(P/F)  FFFFFFFFPPFFPFFFPF

Image AddedImage AddedImage Added

 

Virtual gels from Geneious

 
pENTR CD79A+STOPpENTR CD79A-TCS-Gal4VP16pENTR Syk-eYFPpENTR Syk+STOPpENTR Syk-TEVpENTR Lyn-TEVpENTR Lyn+STOPpENTR Lyn-mKateFail state (ggDONR)
Image AddedImage AddedImage AddedImage AddedImage AddedImage AddedImage AddedImage AddedImage Added
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