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titleName Conventions

For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

...

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

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titleStatus Words
WordMeaning
in planningbeing designed in geneious
designedplasmid design completed in geneious
in labsomeone else in the lab has it
ordered 
in construction 

...

waiting on partdependent on part that is in construction
to be verified 
in storage

we have it in our fridge/freezer, etc

plasmid
NAMEDependency      
GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy

pENTR Gmab Heavy

 pENTR hEF1a

AntibodiesPlanneddesignedexpression      
pEXPR hEF1a: Gmab Light

pENTR Gmab Light

 

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79A

pENTR CD79A+stop

 

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79B+stop

pENTR CD79B+stop

pENTR hEF1a

 

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16 

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16 

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: Lyn+stop

pENTR Lyn+stop

 

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR PTetTRE: Lyn-mKate

pENTR Lyn-mKate

pENTR PTet

 

AntibodiesPlanneddesigned expression      
pEXPR PTetTRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

 

AntibodiesPlanneddesigned expression      
pEXPR PTet: Syk-TEVTEVp

pENTR Syk-TEVp

pENTR PTet

 

AntibodiesPlanneddesigned expression      
pEXPR PTetTRE: eBFP pENTR eBFPAntibodiesIn Freezerin storage?expression      
pEXPR Gal4/UAS: mKate -AntibodiesIn Freezerin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTA pENTR rtTAAntibodiesIn Freezerin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP -Antibodiesin someone else's freezerstorageexpression iGEM -20 CR N/A Velia High 
pEXPR Gal4UAS:mKateTre-TEVp- Antibodiesin someone else's freezerlabexpression      pEXPR Tre-TEVp
pENTR Gmab HeavygBlock mIgM Gmab Heavy Antibodiesin someone else's freezerOrderedentryexpression    IDT  
pENTR Gmab HeavyLightgBlock mIgM Gmab Light AntibodiesOrderedexpressionentry    IDT  
pENTR Gmab LightCD79A (pDONR223)- AntibodiesOrderedin storageexpressionentry iGEM -20   DNASU  
pENTR CD79A+stoppENTR CD79A (pDONR223) Antibodiesin stockconstructionexpressionentry      
pENTR CD79B (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU  
pENTR CD79B+stoppENTR CD79B (pDONR223)Antibodiesin stockconstructionexpressionentry      
pENTR CD79A-TCS-Gal4VP16 pENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		AntibodiesPlannedin constructionexpressionentry      
pENTR CD79B-TCS-Gal4VP16 

pENTR CD79B
pENTR Gal4VP16

AntibodiesPlannedin constructionexpressionentry      
pENTR Lyn (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Lyn+stoppENTR Lyn (pDONR223)Antibodiesin constructionentryAntibodiesIn cultureexpression      
pENTR Lyn-mKatepENTR Lyn
 pENTR mKate		AntibodiesPlannedin constructionexpressionentry      
pENTR Syk (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Syk+stoppENTR Syk (pDONR223)-eYFP AntibodiesPlannedin constructionexpressionentry      
pENTR Syk-TEVeYFPpENTR Syk
pENTR eYFP 		AntibodiesPlannedin constructionexpressionentry      
pENTR Syk-TEVppENTR eBFP Syk
pEXPR Tre-TEVp		Antibodies?in constructionexpressionentry      
pENTR mKateeBFP -Antibodiesin storage?expressionentryiGEM -20 CR N/A Velia High  
pENTR rtTAL1_mKate_L2- Antibodies?in storageexpressionentryiGEM -20 CR N/A Velia High  
pENTR eYFPrtTA -Antibodies?in storageexpressionentry      
pENTR mKate_L1_EYFP_L2- Antibodies?in storageexpressionentry      
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlock   IDT  Ordered from IDT
gBlock mIgM Gmab Light-AntibodiesorderedgBlock   IDT   pENTR Syk (pDONR223)-Antibodiesin culture
pEXPR Tre: BACE2-YFP
  • pENTR L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentin constructionexpressionentry      

pENTR

Lyn (pDONR223)		-Antibodies

L1--BACE2-eYFP--L2

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentin constructionin cultureentry      pENTR CD79A (pDONR223)

Q1-BACE2-Q2

BACE2Treatmentin constructionPCR product-Antibodiesin storageentry      pENTR CD79B (pDONR223)
BACE2n/aTreatmentin the labexpression-Antibodiesin storageentry      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR product      
pEXPR eYFPn/aTreatmentin the labexpression      

pENTR Tre

-TEVp

n/aTreatmentin the labentry      

pDEST R4--R2

n/a Treatmentin the lab alreadydestination      
pEXPR Tre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned expression      

pENTR L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentry      

Q1-BACE1-Q2

BACE1Treatmentplanned PCR product      
BACE1n/aTreatmentin the labexpression Treatment to be ordered    

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR product    eYFP  

pENTR Tre

n/aTreatmentin the lab already entry      

pDEST R4--R2

 n/aTreatmentin the lab already destination      
pEXPR U6:mKate-miRNA(intronic) Treatmentplannedin planning expression      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) Treatmentplannedin planning expression      Experiment 1          
ggDONR

ggDONR L1_LacZ_L2

 Receptorin storageplasmid+4 in iGEM roomLA- Kyle not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough

 

 

ggDONR L1_LacZ_L2 (New)

ggDONR New

 Receptorin storageplasmid+4 in iGEM roomLA-Brandon 
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title...

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

pENTR L4

-

_Hef1a

-

_R1

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector

pDEST

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector 

pEXPR Hef1a:LilrB2

 

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

Receptorin constructionwaiting on partplasmid     waiting on entry vector

pENTR L1-LilrB2-L2

LilrB2 gBlock
    • LilrB2 Entry Vector
 Receptorin constructionto be verified -4 in iGEM roomSS-  
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title
 ifailing
...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

LilrB2 gBlock

 Receptorin storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

pENTR L1_PirB_L2

pENTR L4_Hef1a_R1

  • PirB Expression Vector
 

Receptorin constructionWaiting on partexpression      waiting on entry vector

pENTR L1_PirB_L2

PirB

Entry Vector		 Receptor

gBlock C terminus

PirB gBlock N terminus

Receptorto be verifiedentryin construction     
Expand
title...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212-MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingdesignedin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh