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Excel file
| tube name | Constants Definition | 2-log ladder | pEXPR 1-Hef1a:LacI-2 3 | pEXPR 1-TRE:mKate-2 3 | pEXPR TRE:Gal4VP16 1 | pEXPR TRE:Gal4VP16 2 | |
| gel lane | 0 | 1 | 2 | 3 | 4 | 5 | |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 | |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 | |
| DNA concentration (ng/ul) | 27.6 | 59.6 | 293.3 | 302 | |||
| Enzyme Name | hef1a:laciapaIApaI | SalI | XhoI | XhoI | |||
| Buffer Name | CutSmart | 3.1 | CutSmart | CutSmart | |||
| gel/lane | 0 | 1 | 2 | 3 | 4 | 5 | |
| DNA volume | #DIV/0! | 18.12 | 8.39 | 1.70 | 1.66 | ||
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 | |
| enzyme volume | 1 | 1 | 1 | 1 | 1 | 1 | |
| strip tube # | 1/1 | 1/2 | 1/3 | 1/4 | 1/5 | ||
| water volume | 17.0 | #DIV/0! | -1.1 | 8.6 | 15.3 | 15.3 | |
| gel/lane | 0 | 1 | 2 | 3 | 4 | 5 | |
| loading dye | 4 | 4 | 4 | 4 | 4 | 4 | |
| results | |||||||
| notes |
| tube name | Constants Definition | 2-log ladder | pEXPR 1-Hef1a:LacI-2 3 | pEXPR 1-TRE:mKate-2 3 | pEXPR TRE:Gal4VP16 1 | pEXPR TRE:Gal4VP16 2 |
| gel lane | 0 | 1 | 2 | 3 | 4 | 5 |
| DNA concentration (ng/ul) | 27.6 | 59.6 | 293.3 | 302 | ||
| virtual gel |  |  |  | |||
| notes |
