Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
Comment: Migration of unmigrated content due to installation of a new plugin

...

Expand
titleParts Needed

BCR (CD79A/B, IgM heavy, IgM light), Lyn-mKate

 

anti IgM

Expand
titleSetup

Well 1

HEK293

 

Well 2

HEK293

hEF1a: eBFP

Dummy DNA

Well 3

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

0nM dox

Well 4

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

2000nM dox

Well 5 

 

Well 6 

 

Well 7

HEK 293

Anti-IgM

Well 8

HEK293

hEF1a: eBFP

Dummy DNA

Anti-IgM


Well 9

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

0nM dox

Anti-IgM

Well 10

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

2000nM dox

Anti-IgM

Well 11Well 12

Well 7

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

0nM dox

Well 8

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1nM dox

Well 9

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

10nM dox

Well 10

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

100nM dox

Well 11

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1000nM dox

Well 12

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

2000nM dox

Well 13

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

0nM dox

Anti-IgM

Well 14

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1nM dox

Anti-IgM

Well 15

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

10nM dox

Anti-IgM

Well 16

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

100nM dox

Anti-IgM

Well 17

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1000nM dox

Anti-IgM

Well 18

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

2000nM dox

Anti-IgM

Produce two samples of cells with all parts, anti IgM +/- and look at with a microscope

Expand
titleExpected Results

Lyn should be membrane localized.  We will be lucky if Lyn only membrane localizes in anti IgM+ sampleslocalize in at least some samples. Hopefully Lyn will only localize if the BCR is active and will not localize if it is inactive.

Expand
titleProtocol

 

Expand
titleData

 

...