Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build Choose profile
- Verify sensors
- Create mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Update experiments page
- Obtain plasmid maps
- Email Jin about TAL14 and miRNA target sites
- Talk to Lyla and Alexa about transfection schedule
- Look for plasmids
- UAS-Gal4-TAL14-eYFP
- pEXPR: Gal4VP16
- pEXPR: Hef1a-TAL14
- pEXPR: Hef1a-2x Kturn-eYFP
- Re-analyze all of our data in MATLAB (Gary)
- Learn how to acquire MATLAB from Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Transformations
- Low Sensor Golden Gates
- pEXPR: Hef1a-eBFP2 (186 ng/uL)
- pEXPR: Hef1a-L7ae (96 ng/uL)
- Pick red colonies and grow in medium!
- Find concentrations of eBFP2 and L7ae
NOTES
Nelson doesn't know where it is, but he needs it as well, so he's on the hunt
- Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
- Mini-prep MAV1212 hEF1A Tal14
- Digest and Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3
NOTES:
hEF1a: Gal4VP16 2A rtTA3 culture did not grow so