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titleName Conventions

For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

...

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

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titleStatus Words
WordMeaning
in planningbeing designed in geneious
designedplasmid design completed in geneious
in labsomeone else in the lab has it
ordered 
in construction 

...

waiting on partdependent on part that is in construction
to be verified 
in storage

we have it in our fridge/freezer, etc

WTDNA in freezer, frozen bacteria stock in -80plasmid
NAMEDependency      
GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy

pENTR Gmab Heavy

pENTR hEF1a

AntibodiesPlanneddesignedexpression      
pEXPR hEF1a: Gmab Light

pENTR Gmab Light

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79A

pENTR CD79A+stop

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79B+stop

pENTR CD79B+stop

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR hEF1a: Lyn+stop

pENTR Lyn+stop

pENTR hEF1a

AntibodiesPlanneddesigned expression      
pEXPR PTetTRE: Lyn-mKate

pENTR Lyn-mKate

pENTR PTet

AntibodiesPlanneddesigned expression      
pEXPR PTetTRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

AntibodiesPlanneddesigned expression      
pEXPR PTet: Syk-TEVp

pENTR Syk-

TEV

TEVp

pENTR PTet

AntibodiesPlanneddesigned expression      
pEXPR PTetTRE: eBFPpENTR eBFPAntibodiesIn Freezerin storage?expression      
pEXPR Gal4/UAS: mKate-AntibodiesIn Freezerin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTApENTR rtTAAntibodiesIn Freezerin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Tre-TEVp-Antibodiesin labAntibodyin someone else's freezerexpression      pEXPR Gal4UAS:mKateAntibodyin someone else's freezer
pENTR Gmab HeavygBlock mIgM Gmab HeavyAntibodiesOrderedentryexpression   IDT  
pENTR Gmab LightgBlock mIgM Gmab LightAntibodiesOrderedentry pEXPR Tre-TEVpAntibodyin someone else's freezerexpression   IDT   
pENTR CD79A (pDONR223)Hef1a-rtTaAntibodyAntibodiesin someone else's freezerstorageentryiGEM -20expression  DNASU  
pENTR CD79A+stoppENTR CD79A (pDONR223)Antibodiesin constructionentry  CD79A WT    
pENTR CD79B (pDONR223)-Antibodiesin storageentryiGEM -20Antibodypossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock in -80  DNASU   
pENTR CD79B+stoppENTR CD79B WTAntibodyminiprepped, awaiting sequence verification (pDONR223)Antibodiesin constructionentry DNA in freezer, frozen bacteria stock in -80     
pENTR CD79A-TCS-Gal4VP16AntibodyplannedpENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		Antibodiesin constructionentry       
pENTR CD79B-TCS-Gal4VP16

pENTR CD79B
pENTR Gal4VP16

AntibodyAntibodiesplannedin construction entry      
pENTR Lyn WTAntibodyminiprepped, need to nanodrop and sequence (pDONR223)-Antibodiesin storageentryiGEM -20DNA in freezer, frozen bacteria stock in -80  addgene   
pENTR Lyn+stopLyn-eYFPAntibodypENTR Lyn (pDONR223)Antibodiesin constructionentryplanned       
pENTR Lyn-mKateLyn point mutated (always phosphorylate)AntibodyresearchingpENTR Lyn
pENTR mKate		Antibodiesin constructionentry       
pENTR Syk Antibodyminiprepped, need to nanodrop and sequence (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
 pENTR Syk+stoppENTR Syk -eYFP (mutant non phosphorylatepDONR223)AntibodyAntibodiesplannedin construction entry      
pENTR Syk-TEVpAntibodyeYFPpENTR Syk
pENTR eYFP		Antibodiesin constructionentryplanned       GMab k
pENTR Syk-TEVppENTR Syk
pEXPR Tre-TEVp		Antibodiesin constructionentryAntibodyon order      
pENTR eBFP -GMab HAntibodyAntibodiesin storage?entryon order       GMabH-TCS-Gal4VP16
pENTR L1_mKate_L2-Antibodiesin storageentryAntibodyplanned       
pENTR rtTAhsa-miR-181cmiRNAAntibodiesin storageentryplanned       hsa-miR-9miRNAplanned
pENTR_L1_EYFP_L2-Antibodiesin storageentry       
hsa-miR-30d-5pmiRNAplanned       
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlockhsa-miR-144-5pmiRNAplanned    IDT   
gBlock mIgM Gmab Lighthsa-let-7f-5pmiRNA-AntibodiesorderedgBlockplanned   IDT    has-miR-125bmiRNA
pEXPR Tre: BACE2-YFP
  • pENTR L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentin constructionexpressionplanned      

pENTR L1--BACE2-eYFP--L2

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentin constructionentry has-miR-146amiRNAplanned       
pEXPR TRE

Q1-

t:

BACE2-

eYFP

Q2

BACE2Treatmentin constructionplannedPCR product       
BACE2n/apENTR L4--TRE-t--R1Treatmentin the lab already expression      
pENTR L1

Q2-eYFP-

BACE2-eYFP--L2

QX

pEXPR eYFPTreatmentin the labPCR productplanned       
pEXPR eYFPn/aBACE2 (HsCD00076140)Treatmentin -80the labplasmidexpression AL     eYFP

pENTR Tre

n/aTreatmentin the lab already entry      

pDEST R4--R2

n/aTreatmentin the lab already destination      
pEXPR Hef1a:BACE1-eYFPTre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned  expression      

pENTR

L4--Hef1a--R1		Treatmentin the lab already

L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentry       
pENTR L1

Q1-BACE1-Q2

BACE1-eYFP--L2Treatmentplanned PCR product      
BACE1n/aTreatmentin the labexpressionto be ordered       

Q2-eYFP-QX

pEXPR eYFPTreatmentin the lab alreadyPCR product      

pENTR Tre

n/aTreatmentin the labentry      

pDEST R4--R2

n/aTreatmentin the lab already destination      
pEXPR U6:mKate-miRNA(intronic) Treatmentplannedin planning expression      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)Treatmentplanned  Treatment in planning expression   Experiment 1         

ggDONR L1_LacZ_L2

 ggDONRReceptorin storageplasmid+4 in iGEM roomLA- Kyle 

 

not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough

 

ggDONR L1_LacZ_L2 (New)

 ggDONR NewReceptorin storageplasmid+4 in iGEM roomLA-Brandon 
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title...

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

pENTR L4

-

_Hef1a

-

_R1

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector

pDEST

 Receptorin labplasmid     
Expand
title...

will be used to make LilrB2 expression vector 

pEXPR Hef1a:LilrB2

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

Receptorin constructionwaiting on partplasmid     waiting on entry vector

pENTR L1-LilrB2-L2

LilrB2 Entry VectorgBlockReceptorto be verified in construction-4 in iGEM roomSS-  
Expand
 
title
 ifailing
...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

LilrB2 gBlock

 Receptorin storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

PirB Expression Vector

pENTR L1_PirB_L2

pENTR L4_Hef1a_R1

Receptorin constructionWaiting on partexpression      waiting on entry vector

pENTR L1_PirB_L2

PirB gBlock C terminus

PirB

Entry Vector

gBlock N terminus

Receptorto be verifiedentryin construction     
Expand
title...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh