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titleName Conventions

For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

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pENTR L1-Gene name-L2

pEXPR Promoter:Gene

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titleStatus Words
WordMeaning
in planningbeing designed in geneious
designedplasmid design completed in geneious
in labsomeone else in the lab has it
ordered 
in construction 
waiting on partdependent on part that is in construction
to be verified 
in storage

we have it in our fridge/freezer, etc

NAMEDependency      
GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy

pENTR Gmab Heavy

pENTR hEF1a

Antibodiesdesignedexpression      
pEXPR hEF1a: Gmab Light

pENTR Gmab Light

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79A

pENTR CD79A+stop

pENTR hEF1a

Antibodiesdesigned TypeInitialsConstruction DateLinkExperimentPrioritypEXPR Gal4UAS:eYFPantibodyin someone else's freezerexpression      
pEXPR Gal4UAS:mKateantibodyhEF1a: CD79B+stop

pENTR CD79B+stop

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned pEXPR Tre-TEVpantibodyin someone else's freezerexpression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned Hef1a-rtTaantibodyin someone else's freezerexpression      
pEXPR hEF1a: Lyn+stop

pENTR Lyn+stop

pENTR hEF1a

Antibodiesdesigned expressionCD79A WTantibodyincubating in liquid      CD79B WTantibody
pEXPR TRE: Lyn-mKate

pENTR Lyn-mKate

pENTR PTet

Antibodiesdesigned expressionincubating in liquid      
pEXPR TRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

Antibodiesdesigned expressionCD79A-TCS-Gal4VP16antibodyplanned      CD79B-TCS-Gal4VP16antibody
pEXPR PTet: Syk-TEVp

pENTR Syk-TEVp

pENTR PTet

Antibodiesdesigned expressionplanned      
pEXPR TRE: eBFPpENTR eBFPAntibodiesin storage?expressionLyn WTantibodyincubating on plate      
pEXPR Gal4/UAS: mKate-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTApENTR rtTAAntibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Tre-TEVp-Antibodiesin labexpressionLyn-eYFPantibodyplanned      Lyn point mutated (always phosphorylate)
pENTR Gmab HeavygBlock mIgM Gmab HeavyAntibodiesOrderedentryantibodyresearching   IDT  
pENTR Gmab LightgBlock mIgM Gmab LightAntibodiesOrderedentry Syk WT antibody incubating on plateIDT  
pENTR CD79A (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU  
pENTR CD79A+stoppENTR CD79A (pDONR223)Antibodiesin constructionentrySyk-eYFP (mutant non phosphorylate)antibodyplanned      Syk-TEVpantibody
pENTR CD79B (pDONR223)-Antibodiesin storageentryiGEM -20planned  DNASU  
pENTR CD79B+stoppENTR CD79B (pDONR223)Antibodiesin constructionentry  GMab kantibodyon order      GMab Hantibody
pENTR CD79A-TCS-Gal4VP16pENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		Antibodiesin constructionentryon order      
GMabHpENTR CD79B-TCS-Gal4VP16antibody

pENTR CD79B
pENTR Gal4VP16

Antibodiesin constructionentryplanned      hsa-miR-181cmiRNA
pENTR Lyn (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Lyn+stoppENTR Lyn (pDONR223)Antibodiesin constructionentryplanned      
pENTR Lyn-mKatepENTR Lyn
pENTR mKate		Antibodiesin constructionentryhsa-miR-9miRNAplanned      
pENTR Syk (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Syk+stoppENTR Syk (pDONR223)Antibodiesin constructionentry      
pENTR Syk-eYFPpENTR Syk
pENTR eYFP		Antibodiesin constructionentryhsa-miR-30d-5pmiRNAplanned      hsa-miR-144-5pmiRNA
pENTR Syk-TEVppENTR Syk
pEXPR Tre-TEVp		Antibodiesin constructionentry      
pENTR eBFP-Antibodiesin storage?entryplanned      hsa-let-7f-5p
pENTR L1_mKate_L2-Antibodiesin storageentrymiRNAplanned      
pENTR rtTA-Antibodiesin storageentryhas-miR-125bmiRNAplanned      has-miR-146amiRNA
pENTR_L1_EYFP_L2-Antibodiesin storageentryplanned      
pEXPR TRE-t:BACE2-eYFPhEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlock   IDT  
gBlock mIgM Gmab Light-AntibodiesorderedgBlocktreatmentplanned    IDT  
pEXPR Tre: BACE2-YFP
  • pENTR L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentin constructionexpression
    • pENTR L4--TRE-t--R1
treatmentin the lab already      

pENTR L1--BACE2-eYFP--L2

treatment

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentin constructionentry      

Q1-BACE2-Q2

BACE2Treatmentin constructionPCR productplanned      
BACE2treatmentn/aTreatmentin the labexpression      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR productordered      
pEXPR eYFPn/aTreatmentin the labexpression      

pENTR Tre

n/aTreatment
      • eYFP
treatmentin the lab alreadyentry      

pDEST R4--R2

n/atreatmentTreatmentin the lab alreadydestination      
pEXPR Hef1a:BACE1-eYFPtreatmentTre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned expressionplanned      

pENTR

L4--Hef1a--R1		treatment

L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentryin the lab already      
pENTR L1

Q1-BACE1-Q2

BACE1-eYFP--L2treatmentTreatmentplannedPCR product      
BACE1treatmentn/aTreatmentin the labexpressionto be ordered      

Q2-eYFP

-QX

pEXPR eYFPTreatmentin the labPCR product      

pENTR Tre

n/aTreatmenttreatmentin the lab alreadyentry      

pDEST R4--R2

n/aTreatmenttreatmentin the lab alreadydestination      
pEXPR U6:mKate-miRNA(intronic)treatment Treatmentin planningexpressionplanned      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)treatment Treatmentin planningexpression      

ggDONR L1_LacZ_L2

 Receptorin storageplasmid+4 in iGEM roomLA- Kyle 

 

 

ggDONR L1_LacZ_L2 (New)

 Receptorin storageplasmid+4 in iGEM roomLA-Brandon 
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title...

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

pENTR L4_Hef1a_R1

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector

pDEST

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector 

pEXPR Hef1a:LilrB2

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

Receptorwaiting on partplasmid     waiting on entry vector

pENTR L1-LilrB2-L2

LilrB2 gBlockReceptorto be verified -4 in iGEM roomSS-  
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title...

 failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

LilrB2 gBlock

 Receptorin storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

pENTR L1_PirB_L2

pENTR L4_Hef1a_R1

ReceptorWaiting on partexpression     waiting on entry vector

pENTR L1_PirB_L2

PirB gBlock C terminus

PirB gBlock N terminus

Receptorto be verifiedentry     
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title...

 failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
      planned