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titleName

...

Conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

...

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titleStatus Words
WordMeaning
in planningbeing designed in geneious
designedplasmid design completed in geneious
in labsomeone else in the lab has it
ordered 
in construction 
waiting on partdependent on part that is in construction
to be verified 
in storage

we have it in our fridge/freezer, etc

researching 
NAMEDependency      
GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy

pENTR Gmab Heavy

pENTR hEF1a

Antibodiesdesignedexpression      
pEXPR hEF1a: Gmab Light

pENTR Gmab Light

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79A

pENTR CD79A+stop

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79B+stop

pENTR CD79B+stop

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: Lyn+stop

pENTR Lyn+stop

pENTR hEF1a

Antibodiesdesigned ModuleStatusTypeInitialsConstruction DateLinkExperiment pEXPR Gal4UAS:eYFPantibodyin someone else's freezerexpression      
pEXPR Gal4UASTRE: mKateLyn-mKate

pENTR Lyn-mKate

pENTR PTet

Antibodiesdesigned expression      
pEXPR TRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

Antibodiesdesigned antibodyin someone else's freezerexpression     pEXPR Tre-TEVpantibodyin someone else's freezer 
pEXPR PTet: Syk-TEVp

pENTR Syk-TEVp

pENTR PTet

Antibodiesdesigned expression      
pEXPR TRE: eBFPpENTR eBFPAntibodiesin storage?expression   expression   
pEXPR Gal4/UAS: mKate-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTApENTR rtTAAntibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Tre-TEVp-Antibodiesin labexpression      
pENTR Gmab HeavygBlock mIgM Gmab HeavyAntibodiesOrderedentry Hef1a-rtTaantibodyin someone else's freezerexpression  IDT  
pENTR Gmab LightgBlock mIgM Gmab LightAntibodiesOrderedentry Syk+TEV antibody plannedIDT  
pENTR CD79A (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU  
pENTR CD79A+stoppENTR CD79A (pDONR223)Antibodiesin constructionentry    CD79A WTantibodyincubating in liquid  
pENTR CD79B (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU  
pENTR CD79B+stoppENTR CD79B WT(pDONR223)antibodyAntibodiesincubating in liquidconstructionentry      
pENTR CD79A-TCS-Gal4VP16antibodypENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		Antibodiesin constructionentryplanned      
pENTR CD79B-TCS-Gal4VP16antibodyGal4VP16

pENTR CD79B
pENTR Gal4VP16

Antibodiesin constructionentry    planned  
pENTR Lyn (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Lyn+stoppENTR Lyn WTantibody(pDONR223)Antibodiesin constructionentryincubating on plate      
pENTR Lyn-eYFPantibodymKatepENTR Lyn
pENTR mKate		Antibodiesin constructionentryplanned      Lyn point mutated (always phosphorylate)antibody
pENTR Syk (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
 pENTR Syk+stoppENTR Syk WTantibody(pDONR223)Antibodiesin constructionentryincubating on plate      
pENTR Syk-eYFP (mutant non phosphorylate)antibodypENTR Syk
pENTR eYFP		Antibodiesin constructionentryplanned      
pENTR Syk-TEVppENTR Syk
pEXPR Tre-TEVp		antibodyAntibodiesin constructionentryplanned      
pENTR eBFP-Antibodiesin storage?entryGMab kantibodyon order      GMab Hantibody
pENTR L1_mKate_L2-Antibodiesin storageentryon order      
pENTR rtTA-Antibodiesin storageentryGMabH-TCS-Gal4VP16antibodyplanned      hsa-miR-181c
pENTR_L1_EYFP_L2-Antibodiesin storageentrymiRNAplanned      hsa-miR-9miRNA
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlockplanned    IDT  
gBlock mIgM Gmab Lighthsa-miR-30d-5pmiRNA-AntibodiesorderedgBlockplanned   IDT   hsa-miR-144-5pmiRNA
pEXPR Tre: BACE2-YFP
  • pENTR L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentin constructionexpressionplanned      

pENTR L1--BACE2-eYFP--L2

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentin constructionentryhsa-let-7f-5pmiRNAplanned      
has

Q1-

miR-125b		miRNA

BACE2-Q2

BACE2Treatmentin constructionPCR productplanned      has-miR-146amiRNA
BACE2n/aTreatmentin the labexpressionplanned      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR productpEXPR TRE-t:BACE2-eYFPtreatmentplanned      
pEXPR eYFPn/aTreatment
    • pENTR L4--TRE-t--R1
treatmentin the lab alreadyexpression      

pENTR

L1--BACE2-eYFP--L2		treatment

Tre

n/aTreatmentin the labentryplanned      

pDEST R4--R2

n/aTreatmenttreatmentin the lab alreadydestination      
pEXPR Hef1a:BACE1-eYFPtreatmentTre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned expressionplanned      

pENTR

L4--Hef1a--R1

L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentry      

Q1-BACE1-Q2

BACE1TreatmentplannedPCR product      
BACE1n/aTreatmenttreatmentin the lab alreadyexpression      
pENTR L1

Q2-eYFP-

BACE1-eYFP--L2		treatment

QX

pEXPR eYFPTreatmentin the labPCR product      

pENTR Tre

n/aTreatmentin the labentryplanned      

pDEST R4--R2

n/aTreatmenttreatmentin the lab alreadydestination      
pEXPR U6:mKate-miRNA(intronic)treatment Treatmentin planningexpressionplanned      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)treatment Treatmentin planningexpression      

ggDONR L1_LacZ_L2

 Receptorin storageplasmid+4 in iGEM roomLA- Kyle 

 

 

ggDONR L1_LacZ_L2 (New)

 Receptorin storageplasmid+4 in iGEM roomLA-Brandon 
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title...

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

pENTR L4_Hef1a_R1

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector

pDEST

 Receptorin labplasmid     
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title...

will be used to make LilrB2 expression vector 

pEXPR Hef1a:LilrB2

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

Receptorwaiting on partplasmid     waiting on entry vector

pENTR L1-LilrB2-L2

LilrB2 gBlockReceptorto be verified -4 in iGEM roomSS-  
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title...

 failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

LilrB2 gBlock

 Receptorin storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

pENTR L1_PirB_L2

pENTR L4_Hef1a_R1

ReceptorWaiting on partexpression     waiting on entry vector

pENTR L1_PirB_L2

PirB gBlock C terminus

PirB gBlock N terminus

Receptorto be verifiedentry     
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title...

 failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
      planned