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Results

 

Lane12345678910111213141516
tubeHyper Ladder 1kbGG DONRpENTRSyk-eYFP 6pENTRSyk-eYFP 6pENTRSyk-eYFP 7pENTRSyk-eYFP 8pENTRSyk-eYFP 9pENTRSyk-eYFP 10pENTR Lyn TEV 6pENTR Lyn TEV 6pENTR Lyn TEV 7pENTR Lyn TEV 8pENTR Lyn TEV 9pENTR Lyn TEV 10GG DONRHyper Ladder 1kb
enzyme NsiIundigested controlNsiINsiINsiINsiINsiIundigested controlPvuIIPvuIIPvuIIPvuIIPvuIIPvuII 
Result(P/F)                
Notes                
Lane212223242526272829303132333435
TubeHyper Ladder 1kbGG DONRpENTR Lyn-mKate 6pENTR Lyn-mKate 6pENTR Lyn-mKate 7pENTR Lyn-mKate 8pENTR Lyn-mKate 9pENTR Lyn-mKate 10pENTR Lyn-stop 6pENTR Lyn-stop 6pENTR Lyn-stop 7pENTR Lyn-stop 8pENTR Lyn-stop 9pENTR Lyn-stop 10Hyper Ladder 1kb
Enzyme PvuIIundigested controlPvuIIPvuIIPvuIIPvuIIPvuIIundigested controlPvuIIPvuIIPvuIIPvuIIPvuII 
Result(P/F)               

 

 

Virtual gels from Geneious

pENTR CD79A+STOP
pENTR CD79A-TCS-Gal4VP16
pENTR Syk-eYFP
pENTR Syk+STOP
pENTR Syk-TEV
pENTR Lyn-TEV
pENTR Lyn+STOP
pENTR Lyn-mKate
Fail state (ggDONR)
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