...
| Name | pENTR CD79A+STOP | pENTR CD79A-TCS-Gal4VP16 | pENTR Syk-eYFP | pENTR Syk+STOP | pENTR Syk-TEV | pENTR Lyn-TEV | pENTR Lyn+STOP | pENTR Lyn-mKate | Fail state (ggDONR) |
|---|---|---|---|---|---|---|---|---|---|
| virtual gel |  |  |  |  |  |  |  |  |  |
| selected enzyme | AlwNI | BbsI | BbsI | BbsI | BspQI, SapI | BspHI is bad, looks just like failure mode | AlwNI |