Versions Compared

Old Version 3

changes.mady.by.user Unknown User (gary_9@mit.edu)

Saved on

compared with

New Version 4

changes.mady.by.user Unknown User (gary_9@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
CONSTRUCTION
MAV1212-Hef1a-eBFP2MAV1212-Hef1a-mKate2
  •  Obtain
  •  Gateway together
  •  Golden Gate in Target Sites
  •  Golden Gate in Target Sites
TESTING
  •  Obtain siRNA sequences
  •  Obtain HEK293 cells
  •  Transfect siRNA into HEK cells
  •  Transfect our circuit into HEK cells
  •  Measure fluorescence with flow cytometry