| TASK | WhoDunnit | Observed |
|---|---|---|
| Split cells | AG, LA, SS | |
| Seed plates | AG, LA, SS | |
| Transfection | AG | SS, LA |
| Imaging (FloCyto) |
Practice
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Transfections:
CONTROLS:
- -ve/-ve: no actions performed on cells
- -ve: perform protocol, excluding DNA, or with dummy DNA
- +ve: tri-color control (perform experiments with YFP, BFP, mKATE separately, and one with all 3: allows for comparison of color/intensity when imaging)
- Check for cell death
- Measurement
...
PRACTICE #1: YFP under constitutive promoter
- 6/23: Seed cells
- 6/24: Transfect hEF1a:eYFP into HEK cells
- 6/26 (4.30pm): Flow Cytometer
- Determine optimal Lipofectamine LTX volume (24 well)
- Determine transfection efficiency (quantitatively)
...
PRACTICE #2: _FP under inducible promoter
- 6/26: Cells seeded
- 6/27: Transfect ____ into HEK cells
- 6/29 (__pm): Flow Cytometer
...