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NAME GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy AntibodiesPlannedexpression      
pEXPR hEF1a: Gmab Light AntibodiesPlannedexpression      
pEXPR hEF1a: CD79A AntibodiesPlannedexpression      
pEXPR hEF1a: CD79B AntibodiesPlannedexpression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16 AntibodiesPlannedexpression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16 AntibodiesPlannedexpression      
pEXPR hEF1a: Lyn AntibodiesPlannedexpression      
pEXPR PTet: Lyn-mKate AntibodiesPlannedexpression      
pEXPR PTet: Syk-eYFP AntibodiesPlannedexpression      
pEXPR PTet: Syk-TEV AntibodiesPlannedexpression      
pEXPR PTet: eBFP AntibodiesIn Freezerexpression      
pEXPR Gal4/UAS: mKate AntibodiesIn FreezerexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTA AntibodiesIn FreezerexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP AntibodyAntibodiesin someone else's freezerexpression      
pEXPR Gal4UAS:mKate AntibodyAntibodiesin someone else's freezer

expression

      
pEXPR Tre-TEVp Antibodyin someone else's freezerexpression      Hef1a-rtTa AntibodyAntibodiesin someone else's freezerexpression      
CD79A WT Antibodypossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock in -80     
CD79B WT Antibodyminiprepped, awaiting sequence verification DNA in freezer, frozen bacteria stock in -80     
CD79A-TCS-Gal4VP16 Antibodyplanned       
CD79B-TCS-Gal4VP16 Antibodyplanned       
Lyn WT Antibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
Lyn-eYFP Antibodyplanned       
Lyn point mutated (always phosphorylate) Antibodyresearching       

Syk WT

 Antibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
Syk-eYFP (mutant non phosphorylate) Antibodyplanned       
Syk-TEVp Antibodyplanned       
GMab k Antibodyon order       
GMab H Antibodyon order       
GMabH-TCS-Gal4VP16 Antibodyplanned       
hsa-miR-181c miRNAplanned       
hsa-miR-9 miRNAplanned       
hsa-miR-30d-5p miRNAplanned       
hsa-miR-144-5p miRNAplanned       
hsa-let-7f-5p miRNAplanned       
has-miR-125b miRNAplanned       
has-miR-146a miRNAplanned       
pEXPR TRE-t:BACE2-eYFP Treatmentplanned       
    • pENTR L4--TRE-t--R1
 Treatmentin the lab already       
    • pENTR L1--BACE2-eYFP--L2
 Treatmentplanned       
 Treatmentin -80plasmid AL    
      • eYFP
 Treatmentin the lab already       
    • pDEST R4--R2
 Treatmentin the lab already       
pEXPR Hef1a:BACE1-eYFP Treatmentplanned       
    • pENTR L4--Hef1a--R1
 Treatmentin the lab already       
    • pENTR L1--BACE1-eYFP--L2
 Treatmentplanned       
      • BACE1
 Treatmentto be ordered       
      • eYFP
 Treatmentin the lab already       
    • pDEST R4--R2
 Treatmentin the lab already       
pEXPR U6:mKate-miRNA(intronic) Treatmentplanned       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) Treatmentplanned       
Experiment 1          
  • ggDONR
 Receptor in storageplasmid+4 in iGEM roomLA- Kyle not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
  • ggDONR New
 Receptor in storageplasmid+4 in iGEM roomLA-Brandon will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
  • pENTR L4-Hef1a-R1
 Receptor in labplasmid     will be used to make LilrB2 expression vector
  • pDEST
 Receptor in labplasmid     will be used to make LilrB2 expression vector
  • pEXPR Hef1a:LilrB2
 Receptor in constructionplasmid     waiting on entry vector
    • LilrB2 Entry Vector
 Receptor in constructionplasmid     ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
      • LilrB2 gBlock
 Receptor in storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate
  • PirB Expression Vector
 Receptor in construction      waiting on entry vector
    • PirB Entry Vector
 Receptor in construction      failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
      • PirB gBlock C terminus
 Receptor in storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
      • PirB gBlock N terminus
 Receptor in storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
           
           
           

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