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NAMESTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYOTHER
Antibody Group
pEXPR Gal4UAS:eYFPin someone else's freezerexpression      
pEXPR Gal4UAS:mKatein someone else's freezer

expression

      
pEXPR Tre-TEVpin someone else's freezerexpression      
Hef1a-rtTain someone else's freezerexpression      
CD79A WTminiprepped, awaiting sequence verificationpossibly mislabled DNA and frozen stock  DNA in freezer, frozen bacteria stock     
CD79B WTminiprepped, awaiting sequence verification  DNA in freezer, frozen bacteria stock     
CD79A-TCS-Gal4VP16planned       
CD79B-TCS-Gal4VP16planned       
Lyn WTgrowing in LB, ready at 7amminiprepped, need to nanodrop and sequence  DNA in freezer, frozen bacteria stock     
Lyn-eYFPplanned       
Lyn point mutated (always phosphorylate)researching       

Syk WT

growing in LB, ready at 7amminiprepped, need to nanodrop and sequence  DNA in freezer, frozen bacteria stock     
Syk-eYFP (mutant non phosphorylate)planned       
Syk-TEVpplanned       
GMab kon order       
GMab Hon order       
GMabH-TCS-Gal4VP16planned       
miRNA Group
hsa-miR-181cplanned       
hsa-miR-9planned       
hsa-miR-30d-5pplanned       
hsa-miR-144-5pplanned       
hsa-let-7f-5pplanned       
has-miR-125bplanned       
has-miR-146aplanned       
Treatment Group
pEXPR TRE-t:BACE2-eYFPplanned       
    • pENTR L4--TRE-t--R1
in the lab already       
    • pENTR L1--BACE2-eYFP--L2
planned       
in -80plasmid AL    
      • eYFP
in the lab already       
    • pDEST R4--R2
in the lab already       
pEXPR Hef1a:BACE1-eYFPplanned       
    • pENTR L4--Hef1a--R1
in the lab already       
    • pENTR L1--BACE1-eYFP--L2
planned       
      • BACE1
to be ordered       
      • eYFP
in the lab already       
    • pDEST R4--R2
in the lab already       
pEXPR U6:mKate-miRNA(intronic)planned       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)planned       
Receptor Group
Experiment 1        
  • ggDONR
were not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enoughplasmid+4 in iGEM roomLA- Kyle  
  • ggDONR New
will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stockplasmid+4 in iGEM roomLA-Brandon  
  • Hef1a Entry Vector
in planning - will be used to make LilrB2 expression vectorplasmid      
  • Destination Vector
in planning - will be used to make LilrB2 expression vectorplasmid      
  • LirB2 Expression Vector
in construction - waiting on entry vectorplasmid      
    • LilrB2 Entry Vector
in construction - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNAplasmid      
      • LilrB2 gBlock
used in Golden Gate - in storagegBlock-20 in iGEM roomLA- IDT  
  • PirB Expression Vector
in construction - waiting on entry vector       
    • PirB Entry Vector
in construction - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA       
      • PirB gBlock C terminus
used in Golden Gate - in storagegBlock-20 in iGEM roomLA-IDT  
      • PirB gBlock N terminus
used in Golden Gate - in storage gBlock-20 in iGEM roomLA-IDT  
         
         
         

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