...
- Parts list - look at last year's method of sorting
- Put plasmids on to that list
Plasmid Progress: on Parts List by 3pm:
- miRNA:
- meeting with Jeremy about plasmids for high and low sensors
- Treatment:
- waiting BACE2 + primers
- looking into BACE1 plasmids (pENTR and miRNA)
- TRE + BACE2 plasmid
- BACE2-YFP pENTR (C terminal fusion designed, N terminal fusion should be easy to design)
- Notes
- intronic w/ mKate
- U6 promoter
- U6 teto promoter regulated with tetR
- Antibodies:
- Gantenerumab (Gmab): we have heavy and light chain gBlocks
- CD79A/B: we have pENTR
- Reporter circuit: we have from Valia
- Lyn: we have pENTR
- Look into point mutating it for autophosphorylation
- EYFP fusion
- Syk: we have pENTR
- look into point mutating it
- fusion with EYFP and TEVp
- TEV related plasmids that we have (talk to Nevin):
- Gal4UAS-EYFP
- Gal4UAS-mKate
- TRE-TEVp
- Hef1a-rtTa
- TRE-Syk-TEVp
- TRE-EBFP-TEVp
- G-mab heavy chain - Tcs - Gal4VP16 fusion being designed
- CD79A/B - Tcs - Gal4VP16 being designed
- Receptors:
- Have: pENTR: LilrB2, PirB, Hef1a
- Designed: LilrB2/PirB-EBFP