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- "Overall hairpin length and loop size influence the efficiency of Dicer processing"–how should we design the loop?
- The specificity of base pair matching in the stem influences processing efficiency
- Which of the two strands from the stem is incorporated into the RNA-induced silencing complex?
- miRNA's are readily degraded by RNase (present ubiquitously in cells). So we should be sure to inhibit RNase when working with miRNA's; this inhibitor is available for purchase or might already be in the lab.
Pay attention to cross talk. look for foreign miRNA
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