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  •  Purchase BACE2 plasmid (http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140)
  •  Design primers for BACE2 and EYFP
  •  Run PCR on BACE2 and EYFP
  •  Perform golden gate on PCR products and GGDonr
  •  Perform LR reaction with products from previous step, TRE-t, and backbone
  •  Transfect with expression vector from previous step and rtta vector
  •  Observe fluorescence (more work needed on details of actual experiment)