- Purchase BACE2 plasmid (http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140)
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- Design primers for BACE2 and EYFP
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- Run PCR on BACE2 and EYFP
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- Perform golden gate on PCR products and GGDonr
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- Perform LR reaction with products from previous step and backbone
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- Transfect with expression vector from previous step and rtta vector
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- Observe fluorescence (more work needed on details of actual experiment)
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