hEF1a:eYFP | TRE:eYFP | TRE:mKate | hEF1a:CD79A-RFP-G4 SDM #1 | hEF1a:CD79A-RFP-G4 SDM #2 | hEF1a:CymR | hEF1a-CuO:mKate | CMV5-CuO:mKate | hEF1a:CD79A-3T3-G4 #1 | hEF1a:CD79A-3T9-G4 #1 | hEF1a:CD79A-3T15-G4 #1 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | ||
| Enzyme | StuI | BsaI | BsaI | PvuII | PvuII | PvuII | |||||||
| Virtual gel | Fail
Success bands | Fail
Success bands | |||||||||||
| Concentration (ng/uL) | |||||||||||||
| Buffer | |||||||||||||
| Enzyme | |||||||||||||
| DNA volume | |||||||||||||
| H2O volume | |||||||||||||
| Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | ||
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
...