Versions Compared

Old Version 2

changes.mady.by.user Unknown User (lylaatta@mit.edu)

Saved on

compared with

New Version 3

changes.mady.by.user Unknown User (lylaatta@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

06/17

  • annealed linker oligos
  • golden gate into RFP construct 

06/18

  • transformed golden gate product

06/19

  • all linkers grew colonies except 9TCS6 
  • picked colonies for all except 9TCS6
  • retransformed 9TCS6

06/23 

  • digest and gel verification 
  • 3TCS3, 3TCS9, 9TCSX show correct band pattern

06/24

  • sent 9TCSX for sequencing 

hEF1a:CD79A-XnTCSXc-Gal4VP16

3TCS3 9TCS3 15TCS3
     
 6TCS69TCS612TCS6 
  

...

   
3TCS96TCS99TCS912TCS915TCS9
     
 6TCS129TCS1212TCS12 
     
3TCS15 9TCS15 15TCS15
     

...

annealed linker oligos

golden gate 06/17

...