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June 

Midi
Monday 1stTuesday 2ndWednesday 3rdThursday 4thFriday 5thSaturday 6thSunday 7th
Monday 8thTuesday 9thWednesday 10thThursday 11thFriday 12th Saturday 13thSunday 14th
Monday 15thTuesday 16thWednesday 17thThursday 18thFriday 19thSaturday 20th Sunday 21st
 LR    innoculate midis
 

LR hEF1a:LacI

LR pLac:mKate

LR TRE:mKate

Kill LR + transform

pick colonies

design seq. prim.

miniprep

digest

seq

check seq datainnoculate midis
  

Linker half annealing

GG: CDA-XTCSX-G4

Transform

Pick colonies

Design seq. prim.

Miniprep

Digest

Seq

check seq dataInnoculate midis
    

h:CDB-p:RFP-G4

SDM + gel

  
Monday 22ndTuesday 23rdWednesday 24thThursday 25thFriday 26thSaturday 27thSunday 28th

midiprep

transfection

add small

molecule

cytometry

data analysis

data analysisdata analysis  

Midiprep:

LR hEF1a:LacI

LR pLac:mKate

LR TRE:mKate

transfection

add small

molecule

cytometry 

data analysis

 data analysis

data analyis +

planning for

CD linker

optimization

  
Monday 22ndTuesday 23rdWednesday 24thThursday 25thFriday 26thSaturday 27thSunday 28th
TRE:Gal4VP16 LR

 

TRE:Gal4VP16 transformation

TRE:Gal4VP16 pick colonies TRE:Gal4VP16 mini, digest, seq

analyze seq

TRE:Gal4VP16 midi

 Seed
miniprep, digest, seq

analyze seq

midi

     

CD79A miniprep, digest, seq

CD79B SDM gel

CD79A analyze seq, midi

CD79B linker golden gates

CD79B linker golden gates transform

Syk-RFP-TEV PCRs

CD79B linker golden gates pick colonies

Syk-RFP-TEV golden gate

CD79B linker golden gates mini, digest, seq

Syk-RFP-TEV transform

Syk-RFP-TEV pick colonies 

pLac:Heavy

pLac:Light LRs

pLac:Heavy

pLac:Light transformations

pLac:Heavy

pLac:Light pick colonies

pLac:Heavy

pLac:Light mini, digest, seq

   
Monday 29thTuesday 30th     
transfect+ small molecule     
       

CD79B linker midi

Syk-RFP-TEV mini, digest, seq

      
       


July

check seq dataInnoculate midisMonday 29thTuesday 30th
  Wednesday 1stThursday 2ndFriday 3rdSaturday 4thSunday 5th
 

Transform

h:CDB-p:RFP-G4

pick colonies

design seq. prim.

Miniprep

Digest

Seq

 cytometrydata analysisdata analysis  
       
  Syk-RFP-TEV midi   midiprep 
      
July
Sunday 12th
 
Monday 6thTuesday 7thWednesday 1st8thThursday 2nd9thFriday 3rd10th Saturday 4th11thSunday 5th12th
       Monday 6thTuesday 7thWednesday 8thThursday 9thFriday 10th Saturday 11th
transfect+ small moleculecytometrydata analysisdata analysis  
       
   

pLac:CD79A

pLac:CD79B LR

pLac:CD79A

pLac:CD79B transformations

pLac:CD79A

pLac:CD79B pick colonies

 
Monday 13thTuesday 14thWednesday 15thThursday 16thFriday 17thSaturday 18th Sunday 19th
       
       
transfect+ small moleculecytometrydata analysisdata analysis  

pLac:CD79A

pLac:CD79B mini, digest, seq

 

pLac:CD79A

pLac:CD79B

pLac:Heavy

pLac:Light

midi

    
Monday 20thTuesday 21stWednesday 22ndThursday 23rdFriday 24thSaturday 25thSunday 26th
       
       
       
transfect+small moleculecytometrydata analysisdata analysis  
Monday 27thTuesday 28thWednesday 29thThursday 30th Friday 30th   
       
       
       
       
transfect+ small moleculecytometrydata analysisdata analysis  

August

Float
sideright

Experiments

 Transfection optimization
 Dox/IPTG dose response curve
 
CD79 linker optimization Syk linker
Linker optimization
 Expression level optimization
 Lyn or no
lyn
Lyn