Page History

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1. Team 1 - Grow C. hutch
   1. knowledge base: how-to's for 
      1. liquid + solid cultures
      2. transformation protocols
      3. select
      4. measure growth (Colony Forming Unit (CFU) plates, OD, coulter counter, cytometry)
      5. measure rates of cellulose degradation, rates of byproducts (small organic acids, pentoses/hexoses) production
      6. identify which byproducts affect growth and how
2. Team 2 - Modify C. hutch
   1. Build shuttle vector
      1. plasmids require ori, promoter, genes, restriction sites, selection marker genes
      2. a shuttle vector has everything it need to replicate both in E. coli and C. hutch (need C. hutch promoter, C. hutch ori, C. hutch 
      3. Come up with a map, build a cloning strategy to get there based on the paper
      4. Design, order DNA, and start building (should get done a week before June 8th since the order takes about a week)
3. Team 3 - modify E. Coli
   1. cloning workflow
   2. Circuit design
      1. Modeling (metabolic models, ODEs of growth rates, etc.)
      2. Sequences to put in E. Coli
         1. LuxR, communication
         2. FAEE pathway + sequences (which ones)
   3. Measuring FAEEs - concrete plan
      1. The peaks from the chromatography method may not be distinguishable
      2. extract from cells?
      3. assay development
   4. Measure byproducts that may affect growth