BCR 2.0
Immediate To Do's:
| Task | Claimed by | Date Completed | Other Notes |
|---|---|---|---|
| CD79A/B, Gal4VP16, RFP PCR try3 | CR | Apr 10 |
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| Gel verification of try3 PCR product | LA | Apr 10 | RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers |
| Redesign primers | LA + CR | Apr 11 | CD79A F and R and CD79B F primers were redesigned and ordered |
| AarI golden gate of CD79A construct | LA | Apr 30 | |
| Take out CD79A golden gate plates | LA | ||
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| Redesign CD79B |
| reverse PCR primer |
- task dependent on above task
Higher Level To Do's:
- Finish Discussing Figures for Experiment list
- Gel Extraction of Q1-CD79A-Q3
- Column Purification of pTet-RFP and Gal4VP16 PCR products
- Digest & Gel of CD79A-pTet: RFP - Gal4VP16
- Establish Cryostocks of GmabH, GmabL, Syk-Tev, and Lyn-Tev pEXPRs and dilute DNA to 200 ng/uL
- Decide on other antigen-variable regions we want to test
- Syk-Lyn experiment design
- Receptor dilution experiment design
- Other experimental planning
Useful Links
| Protocols |
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