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Construct
Temperature
CD79A 
CD79B 
Gal4VP16 
pTEt:mRFP 

 

ConstructExpected Band Size
CD79A750 bp
CD79B750 bp
Gal4VP16750 bp
pTet:mRFP800 bp
LadderCD79ACD79BGal4VP16pTet:mRFP

Notes: 

Gal4VP16 constructs have the correct band size --> ready for AarI golden gate

CD79A and CD79B PCRs still seem to have primer dimers. CD79A/B primers have to be redesigned.