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Well 1

HEK293

Well 2

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 3

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 4

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 5

 

Well 6

 

Well 7

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

0nM DOX

Well 8

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

1nM DOX

Well 9

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

10nM DOX

Well 10

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

100nM DOX

Well 11

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

1000nM DOX

Well 12

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

2000nM DOX

Well 13

HEK293

Well 14

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 15

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 16

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 17

 

Well 18

 

Well 19

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

0nM DOX

Well 20

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

1nM DOX

Well 21

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

10nM DOX

Well 22

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

100nM DOX

Well 23

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

1000nM DOX

Well 24

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

2000nM DOX