Page History

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title	Trial 2 Transfection Plan

Well 1 HEK293 (Unstained)	Well 2 HEK293 (Stained)	Well 3 HEK293 hEF1a:LilrB2 (Stained)	Well 4 HEK293 (Unstained)	Well 5 HEK293 (Stained)	Well 6 HEK293 hEF1a:LilrB2 (Stained)
Well 7	Well 8	Well 9	Well 10	Well 11	Well 12
Well 13	Well 14	Well 15	Well 16	Well 17	Well 18
Well 19	Well 20	Well 21	Well 22	Well 23	Well 24

Results:

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title	LilrB2 Trial 1

Microscopy

Permeabilized	Non-permeabilized	Non-permeabilized stained control

Cytometry

Tube 2	Tube 3	Tube 4	Tube 5



Tube 8	Tube 9	Tube 10	Tube 11

Overlay of 4 and 5:

hef1a:mKate + dummy		hef1a:mKate + hef1a:LilrB2
	+		=

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title	Trial 2 Transfection Plan

Well 1 HEK293 (Unstained)	Well 2 HEK293 (Stained)	Well 3 HEK293 hEF1a:PirB (Stained)	Well 4 HEK293 (Unstained)	Well 5 HEK293 (Stained)	Well 6 HEK293 hEF1a:PirB (Stained)
Well 7	Well 8	Well 9	Well 10	Well 11	Well 12
Well 13	Well 14	Well 15	Well 16	Well 17	Well 18
Well 19	Well 20	Well 21	Well 22	Well 23	Well 24

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title	Trial 3 Transfection Plan

In this experiment, we're transfecting the receptor fused to YFP to try get some conclusive results as to whether or not the receptor is localizing to the membrane (since the immunostaining didn't give us any results - or rather gave us weird results)

Well 1 HEK293	Well 2 HEK293 hEF1a:mKate Dummy	Well 3 HEK293 hEF1a:PirB-YFP Dummy	Well 4 HEK293 hEF1a: mKate hEF1a:PirB-YFP	Well 5	Well 6
Well 7 HEK293	Well 8 HEK293 hEF1a:mKate Dummy	Well 9 HEK293 hEF1a:PirB-YFP Dummy	Well 10 HEK293 hEF1a: mKate hEF1a:PirB-YFP	Well 11	Well 12
Well 13	Well 14	Well 15	Well 16	Well 17	Well 18
Well 19	Well 20	Well 21	Well 22	Well 23	Well 24

Results:

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title	PirB Trial 1

Microscopy


Control	Permeabilized	Non-permeabilized	LilrB2 Paper

Cytometry

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title	Gating

SSC-A vs FSC-A	FSC-H vs FSC-W	SSC-H vs SSC-W	Yellow vs Red

Tube 1	Tube 2	Tube 3	Tube 4

Tube 5	Tube 6	Tube 7	Tube 8

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