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  •  Miniprep pEXPR (hEF1a: PirBTCS, hEF1a: TEVpCofilin)
  •  Miniprep pENTR LilrB2TCS
  •  Verify pENTR LilrB2TCS
  •  Midiprep inoculate hEF1a:TCS
  •  Pick more TREt:TEVpCofilin
  •  Plate more TREt:TEVpCofilin if possible.
  •  Transform more TREt:TEVpCofilin LR

...


Digest pEXPR_hEF1a: PirBTCSG4VP16 with BsrGI, buffer 2.1
Digest pENTR_LilrB2:TCSG4VP16 with XhoI +XbaI, buffer CS