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  •  Miniprep PY, LY, pENTR_TEVpCofilin
  •  Verify Minipreps
  •  Transform hEF1a:YFPCofilin
  •  Pick TREt:YFPCofilin Colonies
  •  LR pENTR_TEVpCofilin

 

3/All 4 pENTR_TEVpCofilin worked!

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